Cell Biology Research Associate

Full Time

San Francisco, CA

Job Description

We are seeking a highly-motivated candidate with a strong basic research background in cell biology. The successful candidate will have experience with cell tissue culture, be familiar with conducting routine cell-based assay and comfortable in a fast paced, multitasking environment.

Responsibilities

  • Tissue culture, with the following duties: thaw, grow, passage, count, analyze and freeze cell lines of diverse types; monitor cultures for contamination; use and maintain the cell line record database
  • Plate-based fluorescence and luminescence assays
  • RNA extraction from cultured mammalian cells, PCR, RT-PCR and qPCR
  • Transfection and viral transduction
  • Hands-on experience with common biological/biochemistry research methods including SDS-PAGE, and immunological techniques including ELISA, western blotting, immunofluorescence staining and imaging
  • Coordinate sample analysis among different experiments
  • Maintain timely and detailed records of experiments, results and analysis
  • Ability to identify and troubleshoot critical issues associated with in vitro assays
  • Maintain timely and detailed records of experiments, results and analysis
  • Comply with laboratory safety procedure
  • Perform other laboratory housekeeping tasks as assigned

Skills and requirements

  • BA/BS in biology, Biochemistry, Molecular and Cell Biology, or related scientific field; MS is preferred
  • plus at least two years of laboratory experience
  • Comprehensive understanding of molecular and cell biology principles
  • Demonstrated proficiency to work aseptically in tissue culture
  • Hands on experience in mammalian cell culture, cell-based assay, immunoassay and qPCR are required
  • Attention to detail and careful record keeping skills
  • Ability to multitask
  • Able to work independently
  • Strong computer skills
  • Familiarity with electronic notebook keeping software is desirable

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